A novel role of cyclin-dependent kinase PFTK1 in the differentiation of oligodendrocyte precursor cells

doi:10.3969/j.issn.2095-4344.2015.07.005

Abstract

Abstract:

BACKGROUND: Oligodendrocytes are mainly differentiated from oligodendrocyte precursor cells and the differentiation is tightly regulated by various factors. Some cyclin-dependent kinases play pivotal roles in oligodendrocyte precursor cell differentiation due to their regulation on the early stage of differentiation.

OBJECTIVE: To investigate the potential role of a newly identified cyclin-dependent kinase-like protein, PFTK1 in differentiation and also study the related mechanisms.

METHODS: siRNA technology and western blot assay were used to examine the differentiation markers of OLN-93 and the activation condition of corresponding signal pathways.

RESULTS AND CONCLUSION: With serum-deprivation, OLN-93 underwent differentiation simultaneously. When PFTK1 was silenced by siRNA technology, OLN-93 differentiation was significantly improved, as judged by morphological observations. Accordingly, western blot results showed a marked increase of differentiation markers cyclic nucleotide phosphodiesterase and myelin oligodendrocyte glycoprotein in PFTK1-silenced cells. From the study on PFTK1-related signaling pathways, we found that PFTK1 silencing induced the activation of PI3K/AKT pathway but not MAPK/ERK pathway. Furthermore, the inhibition of AKT by its specific inhibitor abrogated PFTK1 silencing-promoted OLN-93 differentiation. Taken together, our data demonstrate that PFTK1 negatively regulates OLN-93 differentiation through PI3K/AKT pathway, providing a clue for developing new therapeutic approaches to treat degenerative neurological diseases related to demyelination.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Oligodendroglia, Cyclin-Dependent Kinases, Gene Silencing

CLC Number:

R318

Yang Hai-jie, Cui Yang-yang, Wang Lei, Ma Shuang-ping, Li Zhi-chao, Feng Hui-gen. A novel role of cyclin-dependent kinase PFTK1 in the differentiation of oligodendrocyte precursor cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(7): 1008-1012.

Figures/Tables 4

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